Jurnal Kesehatan Rajawali

Perbedaan Hasil Pemeriksaan Sianida Urine dengan Variasi Waktu Penundaan Kadar Awal (1, 2, 4, 8, 24 Jam) yang Disimpan Pada Suhu Ruang dan Rentang Suhu 2-8°C

2025-12-01T07:14:11+00:00

This study aims to determine the differences in the results of cyanide levels in urine stored at room temperature (25°C) and cold temperatures (2–8°C) with variations in the initial delay time (1, 2, 4, 8, and 24 hours). Urine samples that have been added with standard KCN solution were analyzed using a colorimetric test kit. Data were tested using a two-way ANOVA method using Aligned Rank Transformation (ART) followed by post-hoc tests (emmeans and contrast). The results showed that temperature and time factors had a significant effect individually on cyanide levels (p <0.001), but there was no significant interaction between the two (p <0.017511) this value is greater than 0.01 so it can be concluded that there is no significant effect of the interaction between temperature and time on cyanide levels. The highest cyanide levels were recorded at the initial level of 0.0400 ppm and decreased significantly over time to 0.0167 ppm at room temperature. and at cold temperatures it is more capable of maintaining cyanide levels to 0.0267 ppm. Storage at cold temperatures is more capable of maintaining cyanide levels compared to room temperature. These results indicate the importance of temperature management and storage time in maintaining the validity of cyanide examination results in urine.

Gambaran Pemeriksaan Telur Cacing Soil Transmitted Helminths pada Pekerja Sampah di TPA Suwung dengan Pewarnaan Kunyit Kering dan Eosin Metode Direct Slide

2025-12-01T07:14:25+00:00

Soil-Transmitted Helminths (STH) infections are a significant public health issue in tropical regions, particularly among communities with poor sanitation, such as waste workers at the Suwung Landfill in Denpasar. Helminth egg examination is commonly conducted using the direct smear method with eosin staining; however, synthetic dyes have limitations such as high cost and environmental concerns. This study aimed to compare the staining results of STH eggs in fecal preparations using natural dried turmeric dye and eosin through the slide direct method. This research is descriptive in nature, using a qualitative approach. involving 30 STH-positive fecal samples stained using a dried turmeric extract solution (5 g turmeric powder in 20 mL of 96% ethanol) and eosin. Observation parameters included egg color intensity and background contrast. The results showed that staining with dried turmeric revealed 2 positive samples (13.33%), while eosin staining revealed 5 positive samples (33.3%) for STH eggs. The identified helminth species was Ascaris lumbricoides. Eosin was found to be more effective in highlighting the morphology of helminth eggs. However, dried turmeric still shows potential as a supplementary stain due to its accessibility, affordability, and environmental friendliness.

Identifikasi Malassezia furfur Pada Kerokan Kulit Anak-Anak Di Banjar Dinas Batang Karangasem Bali

2025-12-01T07:14:45+00:00

Tinea versicolor is a common superficial fungal infection found in tropical regions such as Indonesia. This condition is often caused by Malassezia furfur, especially in environments with high temperature and humidity and less than optimal hygiene. This study aimed to identify the presence of M. furfur in elementary school children in Banjar Dinas Batang, Karangasem, Bali, a region with a cool, humid climate and limited sanitation access. The study used a descriptive design with a purposive sampling approach, involving 23 children aged 6–18 years who showed skin lesion symptoms and had not undergone antifungal therapy. Skin scrapings were examined microscopically using a 10% KOH solution combined with Parker ink. Observations were made at 100× and 400× magnifications, identifying the distinctive morphology of M. furfur as a “spaghetti and meatballs” structure. Of the 23 samples examined, 13 (56.5%) tested positive for Malassezia furfur. The majority of cases occurred in boys (83%). Hygiene data showed that 61% bathed once a day, 78% changed clothes twice a day, but 87% washed clothes only once a week. Furthermore, most respondents (56%) used rainwater as their source of clean water. The relatively high prevalence of M. furfur infection reflects the strong influence of the humid environment, inadequate sanitation, and suboptimal hygiene behaviors. Microscopic examination using the KOH and Parker ink method proved effective as an initial screening tool in rural areas. Nevertheless, health education interventions and improved access to clean water are needed to reduce the incidence of skin fungal infections in children