Perbedaan Hasil Pemeriksaan Sianida Urine dengan Variasi Waktu Penundaan Kadar Awal (1, 2, 4, 8, 24 Jam) yang Disimpan Pada Suhu Ruang dan Rentang Suhu 2-8°C

Ni Nengah Susi; Nyoman Sudarma; Fairuz Abadi

doi:10.54350/jkr.v15i2.576

Ni Nengah Susi Stikes wira medika bali
Nyoman Sudarma Program Studi Teknologi Laboratorium Medis Program Diploma Tiga, Sekolah Tinggi Ilmu Kesehatan Wira Medika Bali
Fairuz Abadi Program Studi Teknologi Laboratorium Medis Program Diploma Tiga, Sekolah Tinggi Ilmu Kesehatan Wira Medika Bali

DOI: https://doi.org/10.54350/jkr.v15i2.576

Keywords: Cyanide, urine, storage temperature, delay time

Abstract

This study aims to determine the differences in the results of cyanide levels in urine stored at room temperature (25°C) and cold temperatures (2–8°C) with variations in the initial delay time (1, 2, 4, 8, and 24 hours). Urine samples that have been added with standard KCN solution were analyzed using a colorimetric test kit. Data were tested using a two-way ANOVA method using Aligned Rank Transformation (ART) followed by post-hoc tests (emmeans and contrast). The results showed that temperature and time factors had a significant effect individually on cyanide levels (p <0.001), but there was no significant interaction between the two (p <0.017511) this value is greater than 0.01 so it can be concluded that there is no significant effect of the interaction between temperature and time on cyanide levels. The highest cyanide levels were recorded at the initial level of 0.0400 ppm and decreased significantly over time to 0.0167 ppm at room temperature. and at cold temperatures it is more capable of maintaining cyanide levels to 0.0267 ppm. Storage at cold temperatures is more capable of maintaining cyanide levels compared to room temperature. These results indicate the importance of temperature management and storage time in maintaining the validity of cyanide examination results in urine.

References

Abbas, A. (2022). Analisis Deteksi Sianida Menggunakan GC-MS: Akurasi dan Kendala Biaya. Jurnal Forensik dan Toksikologi, 14(2), 87–94.

Anbiyaa, A., & Purnomo, R. (2024). Tanda Klinis dan Forensik Keracunan Sianida pada Kasus Kriminal. Jurnal Kedokteran Forensik Indonesia, 6(1), 41–49.

Arham, N. (2020). Deteksi Sianida dalam Kasus Forensik: Studi Literatur dan Aplikasinya. Jurnal Ilmu Laboratorium Medik, 5(2), 119–127.

Aziza, R., Nugroho, H., & Wulandari, A. (2024). Stabilitas Kadar Sianida Urine pada Berbagai Suhu Penyimpanan. Jurnal Biomedika dan Laboratorium, 8(1), 54–60.

Dewi, S. A., Prasetyo, H., & Susanti, R. (2023). Paparan dan Deteksi Sianida dalam Lingkungan Industri. Jurnal Kesehatan Lingkungan, 12(3), 201–210.

Ekinci, M., Kaya, M., & Yilmaz, M. (2019). Cyanide Poisoning and Antidote Administration: A Critical Review. Journal of Emergency Toxicology, 28(4), 233–240.

Fonataba, F. (2022). Kit Uji Kolorimetri Sianida: Solusi Deteksi Cepat di Lapangan. Jurnal Farmasi dan Toksikologi, 10(2), 88–96.

Girgin, D. (2023). A Comparison of Photometric and Colorimetric Cyanide Detection Kits. International Journal of Clinical Chemistry, 39(3), 155–160.

Majalis, T. (2022). Risiko Paparan Sianida dalam Proses Industri Pelapisan Logam. Jurnal Keselamatan Kerja Indonesia, 7(1), 21–30.

Nantika, Y. (2017). Stabilitas Sianida dalam Sampel Biologis: Studi Literatur. Jurnal Toksikologi dan Laboratorium Medik, 3(1), 33–39.

Nath, K. (2017). Analytical Techniques for Determining Cyanide in Biological Fluids. Analytical Toxicology Journal, 45(1), 62–71.

Pitoi, E. (2015). Volatilisasi dan Degradasi Sianida: Pengaruh Suhu dan pH. Jurnal Kimia Dasar dan Terapan, 9(2), 142–149.

Rahmawati, L. (2024). Stabilitas Sianida pada Suhu Penyimpanan Berbeda: Implikasi Forensik. Jurnal Toksikologi Klinis Indonesia, 11(1), 14–20.

Sulistyarti, H. (2015). Efek Toksik Sianida terhadap Sistem Respirasi Seluler. Jurnal Biokimia Indonesia, 6(1), 27–35.

Tusiewicz, A., Lewandowska, A., & Wszolek, K. (2024). Cyanide Stability in Long-term Frozen Blood Storage: A Case Study. Journal of Analytical Toxicology, 48(1), 15–21.

Widyastani, S. (2023). Efek Paparan Sianida terhadap Sistem Pernapasan dan Sirkulasi. Jurnal Toksikologi dan Kesehatan, 12(2), 98–104.

Yuanita, L. (2020). Ketidakstabilan Sianida dan Dampaknya terhadap Prosedur Pemeriksaan Laboratorium. Jurnal Ilmu Laboratorium Medik, 6(2), 73–80.